A streamlined collecting and preparation protocol for DNA barcoding of Lepidoptera as part of large-scale rapid biodiversity assessment projects, exemplified by the Indonesian Biodiversity Discovery and Information System (IndoBioSys)
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A streamlined collecting and preparation protocol for DNA barcoding of Lepidoptera as part of large-scale rapid biodiversity assessment projects, exemplified by the Indonesian Biodiversity Discovery and Information System (IndoBioSys)
- by
- Schmidt, Olga; Hausmann, Axel; Cancian de Araujo, Bruno; Sutrisno, Hari; Peggie, Djunijanti; Schmidt, Stefan
- Publication date
- 2017-10-31
- Topics
- Collecting methods, DNA barcoding, light trap, monitoring, moths, rapid biodiversity assessment, sampling protocol
- Publisher
- Pensoft Publishers
- Collection
- biodiversity
- Contributor
- Pensoft Publishers
- Language
- English
- Rights-holder
- Copyright held by individual article author(s).
- Volume
- 5
- Item Size
- 23.0M
- Abstract
- Here we present a general collecting and preparation protocol for DNA barcoding of Lepidoptera as part of large-scale rapid biodiversity assessment projects, and a comparison with alternative preserving and vouchering methods. About 98% of the sequenced specimens processed using the present collecting and preparation protocol yielded sequences with more than 500 base pairs. The study is based on the first outcomes of the Indonesian Biodiversity Discovery and Information System (IndoBioSys). IndoBioSys is a German-Indonesian research project that is conducted by the Museum für Naturkunde in Berlin and the Zoologische Staatssammlung München, in close cooperation with the Research Center for Biology – Indonesian Institute of Sciences (RCB-LIPI, Bogor).
- Addeddate
- 2025-01-21 23:18:44
- Bhl_virtual_titleid
- 210877
- Bhl_virtual_volume
- v.5 (2017)
- Call number
- 10_3897_BDJ_5_e20006
- Call-number
- 10_3897_BDJ_5_e20006
- Foldoutcount
- 0
- Genre
- article
- Identifier
- streamlinedcoll5schm
- Identifier-ark
- ark:/13960/s27z3c9rm4s
- Identifier-bib
- 10_3897_BDJ_5_e20006
- Identifier-doi
- 10.3897/BDJ.5.e20006
- Identifier-elocator
- e20006
- Ocr
- tesseract 5.3.0-6-g76ae
- Ocr_detected_lang
- en
- Ocr_detected_lang_conf
- 1.0000
- Ocr_detected_script
- Latin
- Ocr_detected_script_conf
- 1.0000
- Ocr_module_version
- 0.0.21
- Ocr_parameters
- -l eng
- Page_number_confidence
- 91
- Page_number_module_version
- 1.0.5
- Page_range
- 1-14
- Pages
- 14
- Pdf_degraded
- invalid-jp2-headers
- Pdf_module_version
- 0.0.25
- Possible copyright status
- In copyright. Digitized with the permission of the rights holder.
- Ppi
- 300
- Source
- Biodiversity Data Journal 5
- Year
- 2017
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